Are you preparing for the RRB Group D 2025 exam? Biology-based General Science questions play a crucial role in scoring well in the CBT. To help you strengthen your preparation, we’ve compiled 20 of the most important and frequently asked biology-related multiple-choice questions along with their answers.
These questions cover topics like genetic engineering, medical diagnostics, biotechnology, and plant science—aligned with the latest syllabus and previous year trends.


Expected Biology Questions for RRB Group D 2025
Here are the Answers:
1. With the help of genetic engineering, ‘Golden Rice’ is enriched with:
a. Vitamin A and nicotinic acid
b. Beta-carotene, Vitamin A, and folic acid
c. Beta-carotene and iron
d. Vitamin A and niacin
2. Which of the following is used as a biofertilizer during rice cultivation?
a. Blue-green algae
b. Rhizobium
c. Fungi
d. Azotobacter
3. Yeast is used in bakeries to make bread because:
a. It makes the bread hard
b. It makes the bread soft and spongy
c. It enhances the nutritional value of the bread
d. It keeps the bread fresh
4. Who developed the contact lens sensor that can monitor indicators like glaucoma, stress hormone, uric acid, and pressure sensing?
a. Professor Keith
b. Professor Greg Harmon
c. Professor Sensor
d. Professor Blake William
5. What is meant by angiogram?
a. A medical diagnostic test
b. A signal used in the telegram office
c. A signal used in radio
d. A signal used in television
6. In which year was the first human heart transplant performed?
a. 1947
b. 1957
c. 1967
d. 1977
7. ‘Golden Rice’ is enriched with:
a. Vitamin A
b. Vitamin C
c. Vitamin E
d. Vitamin D
8. In the case of improved crop Bt-cotton, the gene is transferred from:
a. Virus
b. Bacteria
c. Fungi
d. Algae
9. Using recombinant DNA technology, genes can be transferred to:
a. Only plants
b. From animals to plants
c. From microbes to higher organisms
d. All of the above
10. Which enzyme is referred to as “molecular scissors” and is used to cut DNA at specific locations in genetic engineering?
a. DNA ligase
b. DNA polymerase
c. Restriction endonuclease
d. Restriction exonuclease
11. Bt-seed is related to:
a. Rice
b. Wheat
c. Cotton
d. Oilseeds
Also Read: RRB Group D Mock Drill 2025: Boost Accuracy with These Practice MCQs
12. A variety of brinjal developed using genetic engineering is called Bt-brinjal. What was the purpose of this development?
a. To protect from insects
b. To improve taste and nutrition
c. To make it drought-resistant
d. To increase shelf life
13. Which of the following is NOT a medical diagnostic test?
a. Colonoscopic test
b. McNaughton test
c. Doppler test
d. CT scan
14. What does an electrocardiogram (ECG) measure?
a. Leg movements
b. Blood circulation
c. Brain cells
d. Heartbeats
15. What is a “magic bullet”?
a. A bullet that kills people
b. A drug that targets only one specific disease without side effects
c. A missile
d. A powerful microscope
16. Why is a silica gel pouch often placed in capsule or tablet bottles?
a. To kill bacteria
b. To absorb bacteria
c. To keep the bottle warm
d. To absorb moisture
17. Which type of radiation is most responsible for causing cell mutation and cancer?
a. Beta rays
b. Alpha rays
c. Gamma rays
d. X-rays
18. From the plant Rosy Periwinkle, a medicine is derived to treat:
a. Malaria
b. Blood cancer
c. High blood pressure
d. Anemia
19. What is the purpose behind developing a genetically engineered variety of brinjal?
a. To enhance taste and nutrition
b. To make it pest-resistant
c. To make it drought-resistant
d. To increase its shelf life
20. Aspirin is a:
a. Antibiotic
b. Antiseptic
c. Antipyretic
d. None of the above
Also Read: RRB NTPC Mock Drill 2025: Practice Test Series, Free Quizzes & Preparation Tips
Answers to the Expected Biology Questions for RRB Group D 2025
Here are the answers:
- b
- a
- b
- b
- a
- c
- a
- b
- d
- c
- c
- a
- b
- d
- b
- d
- c
- b
- b
- c